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PLCG2 Polyclonal antibody

PLCG2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27173-1-AP

Synonyms

Phospholipase C gamma 2, Phospholipase C IV, PLC gamma 2, PLC IV, PLC γ2, PLCG2, PLCγ2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLCG2 Polyclonal antibody (27173-1-AP)

    WB analysis using 27173-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27173-1-AP (PLCG2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PLCG2 Polyclonal antibody (27173-1-AP)

    IHC staining of human tonsillitis using 27173-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27173-1-AP (PLCG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PLCG2 Polyclonal antibody (27173-1-AP)

    IHC staining of human tonsillitis using 27173-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27173-1-AP (PLCG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27173-1-AP targets PLCG2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLCG2 fusion protein Ag25847
Full Name phospholipase C, gamma 2 (phosphatidylinositol-specific)
Calculated Molecular Weight 148 kDa
Observed Molecular Weight 148 kDa
GenBank Accession NumberBC007565
Gene Symbol PLCG2
Gene ID (NCBI) 5336
RRIDAB_2880785
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PLCG2 antibody 27173-1-APDownload protocol
IHC protocol for PLCG2 antibody 27173-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols