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  • Featured Product
  • KD/KO Validated

Plexin B2 Polyclonal antibody

Plexin B2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10602-1-AP

Synonyms

KIAA0315, MM1, Nbla00445, PLEXB2, Plexin B2, PLXNB2

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using Plexin B2 Polyclonal antibody (10602-1-AP)

    WB analysis of A549 using 10602-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Plexin B2 Polyclonal antibody (10602-1-AP)

    WB analysis of human brain using 10602-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Plexin B2 Polyclonal antibody (10602-1-AP)

    WB analysis of HEK-293 using 10602-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Plexin B2 Polyclonal antibody (10602-1-AP)

    WB analysis of HeLa using 10602-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Plexin B2 Polyclonal antibody (10602-1-AP)

    WB analysis of human brain using 10602-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using Plexin B2 Polyclonal antibody (10602-1-AP)

    WB analysis of mouse liver using 10602-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using Plexin B2 Polyclonal antibody (10602-1-AP)

    WB analysis of mouse ovary using 10602-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Plexin B2 Polyclonal antibody (10602-1-AP)

    WB analysis of MCF-7 using 10602-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Plexin B2 Polyclonal antibody (10602-1-AP)

    WB analysis of HepG2 using 10602-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using Plexin B2 Polyclonal antibody (10602-1-AP)

    IP experiment of mouse liver using 10602-1-AP

    IP result of anti-Plexin B2 (IP:10602-1-AP, 4ug; Detection:10602-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Plexin B2 Polyclonal antibody (10602-1-AP)

    IHC staining of human tonsillitis using 10602-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Plexin B2 Polyclonal antibody (10602-1-AP)

    IHC staining of human tonsillitis using 10602-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Plexin B2 Polyclonal antibody (10602-1-AP)

    IHC staining of human liver cancer using 10602-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Plexin B2 Polyclonal antibody (10602-1-AP)

    IHC staining of human liver cancer using 10602-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Plexin B2 Polyclonal antibody (10602-1-AP)

    IHC staining of human breast cancer using 10602-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Plexin B2 Polyclonal antibody (10602-1-AP)

    IHC staining of human breast cancer using 10602-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Plexin B2 Polyclonal antibody (10602-1-AP)

    IHC staining of human gliomas using 10602-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Plexin B2 Polyclonal antibody (10602-1-AP)

    IHC staining of human gliomas using 10602-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Plexin B2 Polyclonal antibody (10602-1-AP)

    IF Staining of HepG2 using 10602-1-AP

    Immunofluorescent analysis of HepG2 cells using 10602-1-AP (Plexin B2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, MCF-7 cells, mouse liver tissue, mouse ovary tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman tonsillitis tissue, human liver cancer tissue, human breast cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10602-1-AP targets Plexin B2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Plexin B2 fusion protein Ag0941
Full Name plexin B2
Calculated Molecular Weight 205 kDa
Observed Molecular Weight 80 kDa, 240 kDa
GenBank Accession NumberBC004542
Gene Symbol PLXNB2
Gene ID (NCBI) 23654
RRIDAB_2166424
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB2 is one of the three plexin-B family members which participate in axon guidance and cell migration in response to semaphorins. It has been reported that plexin B2 precursor is 240 kDa and can be cleaved into alpha subunit (~170-kDa) and beta subunit (80-kDa) (PMID: 12533544). This antibody raised against 1542-1841aa of human plexin B2 can recognize precursor and beta subunit of plexin B2.

Protocols

Product Specific Protocols
WB protocol for Plexin B2 antibody 10602-1-APDownload protocol
IHC protocol for Plexin B2 antibody 10602-1-APDownload protocol
IF protocol for Plexin B2 antibody 10602-1-APDownload protocol
IP protocol for Plexin B2 antibody 10602-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Osteoclasts protect bone blood vessels against senescence through the angiogenin/plexin-B2 axis.

Authors - Xiaonan Liu
  • KD Validated
View Article
mouseWB,IF

EMBO J

Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis.

Authors - Rongpan Bai
  • KD Validated
View Article
humanWB,IHC,IP

bioRxiv

Computational ranking-assisted identification of Plexin-B2 in homotypic and heterotypic clustering of circulating tumor cells in breast cancer metastasis

Authors - Emma Schuster
  • KD Validated
View Article
mouseIF

Sci Adv

A balance of noncanonical Semaphorin signaling from the cerebrospinal fluid regulates apical cell dynamics during corticogenesis

Authors - Katrin Gerstmann
View Article
mouseIF

Hum Reprod

Whole exome sequencing in unexplained recurrent miscarriage families identified novel pathogenic genetic causes of euploid miscarriage

Authors - Xiyao Wang
View Article
mouseWB

Mol Hum Reprod

Semaphorin 4C regulates ovarian steroidogenesis through RHOA/ROCK1-mediated actin cytoskeleton rearrangement

Authors - Dan Chen
View Article