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PLXNB2 Monoclonal antibody

PLXNB2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2G9B2

Cat no : 67265-1-Ig

Synonyms

dJ402G11.3, KIAA0315, MM1, Nbla00445, PLEXB2, plexin B2, PLXNB2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLXNB2 Monoclonal antibody (67265-1-Ig)

    WB analysis using 67265-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of ATDC-5 cells using PLXNB2 Monoclonal antibody (67265-1-Ig)

    WB analysis of ATDC-5 using 67265-1-Ig

    ATDC-5 cells were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using PLXNB2 Monoclonal antibody (67265-1-Ig)

    WB analysis of U2OS using 67265-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PLXNB2 Monoclonal antibody (67265-1-Ig)

    WB analysis using 67265-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PLXNB2 Monoclonal antibody (67265-1-Ig)

    IHC staining of mouse brain using 67265-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67265-1-Ig (PLXNB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PLXNB2 Monoclonal antibody (67265-1-Ig)

    IHC staining of mouse brain using 67265-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67265-1-Ig (PLXNB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inMDA-MB-231 cells, rat brain tissue, ATDC-5 cells, U2OS cells, Mouse brain tissue, MCF-7 cells, A549 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67265-1-Ig targets PLXNB2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLXNB2 fusion protein Ag28706
Full Name plexin B2
Calculated Molecular Weight 205 kDa
Observed Molecular Weight 240 kDa
GenBank Accession NumberNM_012401
Gene Symbol PLXNB2
Gene ID (NCBI) 23654
RRIDAB_2882536
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB2 is one of the three plexin-B family members which participate in axon guidance and cell migration in response to semaphorins. It has been reported that plexin B2 precursor is 240 kDa and can be cleaved into alpha subunit (~170-kDa) and beta subunit (80-kDa) (PMID: 12533544). This antibody raised against 20-466aa of human plexin B2 can recognize precursor and alpha subunit of plexin B2.

Protocols

Product Specific Protocols
WB protocol for PLXNB2 antibody 67265-1-IgDownload protocol
IHC protocol for PLXNB2 antibody 67265-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols