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POLA2 Polyclonal antibody

POLA2 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21778-1-AP

Synonyms

DNA polymerase alpha subunit B, POLA2

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using POLA2 Polyclonal antibody (21778-1-AP)

    WB analysis of HepG2 using 21778-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using POLA2 Polyclonal antibody (21778-1-AP)

    WB analysis of COLO 320 using 21778-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using POLA2 Polyclonal antibody (21778-1-AP)

    WB analysis of HeLa using 21778-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using POLA2 Polyclonal antibody (21778-1-AP)

    WB analysis of HepG2 using 21778-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using POLA2 Polyclonal antibody (21778-1-AP)

    WB analysis of HeLa using 21778-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using POLA2 Polyclonal antibody (21778-1-AP)

    WB analysis of HeLa using 21778-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using POLA2 Polyclonal antibody (21778-1-AP)

    WB analysis of COLO 320 using 21778-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using POLA2 Polyclonal antibody (21778-1-AP)

    WB analysis of MCF-7 using 21778-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using POLA2 Polyclonal antibody (21778-1-AP)

    IF Staining of HeLa using 21778-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21778-1-AP (POLA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, COLO 320 cells, HeLa cells, MCF-7 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21778-1-AP is POLA2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLA2 fusion protein Ag0271
Full Name polymerase (DNA directed), alpha 2 (70kD subunit)
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 68-70 kDa
GenBank Accession NumberBC001347
Gene Symbol POLA2
Gene ID (NCBI) 23649
RRIDAB_10733243
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for POLA2 antibody 21778-1-APDownload protocol
IF protocol for POLA2 antibody 21778-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

CST does not evict elongating telomerase but prevents initiation by ssDNA binding.

Authors - Arthur J Zaug
View Article
humanIHC

Cancer Cell Int

Overexpression of POLA2 in hepatocellular carcinoma is involved in immune infiltration and predicts a poor prognosis

Authors - Long Liu
View Article