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OCT2 Polyclonal antibody

OCT2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10867-2-AP

Synonyms

Oct 2, OCT2, Octamer binding protein 2, OTF 2, OTF2, POU class 2 homeobox 2, POU2F2

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using OCT2 Polyclonal antibody (10867-2-AP)

    WB analysis of NIH/3T3 using 10867-2-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10867-2-AP (OCT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using OCT2 Polyclonal antibody (10867-2-AP)

    WB analysis of mouse ovary using 10867-2-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10867-2-AP (OCT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using OCT2 Polyclonal antibody (10867-2-AP)

    IP experiment of NIH/3T3 using 10867-2-AP

    IP result of anti-OCT2 (IP:10867-2-AP, 4ug; Detection:10867-2-AP 1:300) with NIH/3T3 cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using OCT2 Polyclonal antibody (10867-2-AP)

    IHC staining of human lymphoma using 10867-2-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 10867-2-AP (OCT2 antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inNIH/3T3 cells, mouse ovary tissue
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10867-2-AP targets OCT2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OCT2 fusion protein Ag0955
Full Name POU class 2 homeobox 2
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC006101
Gene Symbol POU2F2
Gene ID (NCBI) 5452
RRIDAB_2167080
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POU2F2, also named as OCT2, is a 479 amino acid protein, which belongs to the POU transcription factor family. The Pit-Oct-Unc (POU) family of transcription factors includes many proteins that have been grouped in seven distinct classes according to function and expression containing proteins such as the neuronally expressed Pit proteins in class I, the ubiquitously expressed Oct-1 and tissue-specific Oct-2 in the second category, the well-known pluripotency marker Oct-4 which belongs to class V. Oct-1 (POU2f1) is a ubiquitous factor present in a wide range of cell types while Oct-2 (POU2f2) expression has been found confined to B lymphocytes and neuronal cells. Oct-2 has been implicated in B cell development and regulation of B lymphocyte-specific genes. POU2F2 exists various isoforms with MV 43, 50, and 62 kDa.

Protocols

Product Specific Protocols
WB protocol for OCT2 antibody 10867-2-APDownload protocol
IHC protocol for OCT2 antibody 10867-2-APDownload protocol
IP protocol for OCT2 antibody 10867-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols