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PPIL6 Polyclonal antibody

PPIL6 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 17452-1-AP

Synonyms

Cyclophilin like protein PPIL6, PPIase, PPIL6, Rotamase PPIL6

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PPIL6 Polyclonal antibody (17452-1-AP)

    WB analysis of HeLa using 17452-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17452-1-AP (PPIL6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PPIL6 Polyclonal antibody (17452-1-AP)

    WB analysis of HepG2 using 17452-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17452-1-AP (PPIL6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PPIL6 Polyclonal antibody (17452-1-AP)

    IF Staining of HeLa using 17452-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17452-1-AP (PPIL6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

17452-1-AP targets PPIL6 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPIL6 fusion protein Ag11578
Full Name peptidylprolyl isomerase (cyclophilin)-like 6
Calculated Molecular Weight 311 aa, 35 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC038716
Gene Symbol PPIL6
Gene ID (NCBI) 285755
RRIDAB_2237569
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PPIL6 antibody 17452-1-APDownload protocol
IF protocol for PPIL6 antibody 17452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Curr Biol

CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement

Authors - Chao Liu
View Article
mouseWB

Cell Mol Life Sci

CCDC146 is required for sperm flagellum biogenesis and male fertility in mice

Authors - Yanjie Ma
View Article