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PPP2R1B Polyclonal antibody

PPP2R1B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

Cat no : 12621-1-AP

Synonyms

PP2A subunit A isoform R1 beta, PPP2R1B, PR65B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP2R1B Polyclonal antibody (12621-1-AP)

    WB analysis using 12621-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12621-1-AP (PPP2R1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PPP2R1B Polyclonal antibody (12621-1-AP)

    WB analysis using 12621-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12621-1-AP (PPP2R1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using PPP2R1B Polyclonal antibody (12621-1-AP)

    WB analysis of human kidney using 12621-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12621-1-AP (PPP2R1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using PPP2R1B Polyclonal antibody (12621-1-AP)

    IHC staining of human liver using 12621-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12621-1-AP (PPP2R1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using PPP2R1B Polyclonal antibody (12621-1-AP)

    IHC staining of human liver using 12621-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12621-1-AP (PPP2R1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using PPP2R1B Polyclonal antibody (12621-1-AP)

    IF Staining of Caco-2 using 12621-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using PPP2R1B antibody (12621-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA2780 cells, human kidney tissue, mouse testis tissue, rat testis tissue, HeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12621-1-AP targets PPP2R1B in WB,IHC,IF,ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP2R1B fusion protein Ag3307
Full Name protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform
Calculated Molecular Weight 667 aa, 74 kDa
Observed Molecular Weight 60-74 kDa
GenBank Accession NumberBC027596
Gene Symbol PPP2R1B
Gene ID (NCBI) 5519
RRIDAB_2169941
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PPP2R1B antibody 12621-1-APDownload protocol
IHC protocol for PPP2R1B antibody 12621-1-APDownload protocol
IF protocol for PPP2R1B antibody 12621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells.

Authors - Baoyu Chen
View Article
mouseWB

EMBO Rep

CIP2A deficiency promotes depression-like behaviors in mice through inhibition of dendritic arborization

Authors - Wen-Ting Hu
View Article
human,mouseWB,IHC,CoIP

Cancer Cell Int

PPP2R1B abolishes colorectal cancer liver metastasis and sensitizes Oxaliplatin by inhibiting MAPK/ERK signaling pathway

Authors - Wei Liu
  • KD Validated
View Article