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PRAM1 Polyclonal antibody

PRAM1 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 13651-1-AP

Synonyms

PML RAR, PRAM 1, PRAM1

Validation Data Gallery

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  • Western Blot (WB) analysis of U-937 cells using PRAM1 Polyclonal antibody (13651-1-AP)

    WB analysis of U-937 using 13651-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 13651-1-AP (PRAM1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

Tested Applications

Positive WB detected inU-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13651-1-AP targets PRAM1 in WB,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRAM1 fusion protein Ag4162
Full Name PML-RARA regulated adaptor molecule 1
Calculated Molecular Weight 718 aa, 79 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC028012
Gene Symbol PRAM1
Gene ID (NCBI) 84106
RRIDAB_2877964
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRAM1, also named as PML-RARA-regulated adapter molecule 1, is a 718 amino acid protein, which contains 1 SH3 domain. PRAM1 is expressed in peripheral blood leukocytes and bone marrow. It is not expressed in non hematopoietic tissues except in lung. PRAM1 may be involved in myeloid differentiation and integrin signaling in neutrophils.

Protocols

Product Specific Protocols
WB protocol for PRAM1 antibody 13651-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols