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PRR14 Polyclonal antibody

PRR14 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 22819-1-AP

Synonyms

proline rich 14, Proline rich protein 14, PRR14

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRR14 Polyclonal antibody (22819-1-AP)

    WB analysis using 22819-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22819-1-AP (PRR14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using PRR14 Polyclonal antibody (22819-1-AP)

    WB analysis of HL-60 using 22819-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 22819-1-AP (PRR14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PRR14 Polyclonal antibody (22819-1-AP)

    WB analysis of mouse brain using 22819-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22819-1-AP (PRR14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHCT 116 cells, HL-60 cells, MCF-7 cells, SW480 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22819-1-AP is PRR14 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRR14 fusion protein Ag14119
Full Name proline rich 14
Calculated Molecular Weight 585 aa, 64 kDa
Observed Molecular Weight 64-70 kDa
GenBank Accession NumberBC000119
Gene Symbol PRR14
Gene ID (NCBI) 78994
RRIDAB_2879174
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRR14 (proline-rich protein 14) is a 585-amino acid protein with a calculated molecular weight of 64 kDa. This protein functions to tether heterochromatin to the nuclear periphery during interphase, through associations with heterochromatin protein 1 (HP1) and the nuclear lamina. After disassembly early in mitosis, PRR14 reassembles in two steps, first binding to anaphase chromosomes through HP1, followed by association with the nuclear lamina in telophase. PRR14 may thereby play a role in specifying HP1-bound heterochromatin for reattachment to the nuclear lamina at mitotic exit. (PMID: 24209742; 24637393)

Protocols

Product Specific Protocols
WB protocol for PRR14 antibody 22819-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Nucleus

PRR14 organizes H3K9me3-modified heterochromatin at the nuclear lamina

Authors - Anna A Kiseleva
  • KD Validated
View Article