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PSG1 Polyclonal antibody

PSG1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11809-1-AP

Synonyms

B1G1, CD66f, DHFRP2, FL NCA 1/2, PBG1, PS beta C/D, PS beta G 1, PSBG 1, PSBG1, PSG1, PSG95, PSGGA, PSGIIA, SP1

Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using PSG1 Polyclonal antibody (11809-1-AP)

    WB analysis of human kidney using 11809-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11809-1-AP (PSG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PSG1 Polyclonal antibody (11809-1-AP)

    WB analysis of MCF-7 using 11809-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11809-1-AP (PSG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PSG1 Polyclonal antibody (11809-1-AP)

    IHC staining of human placenta using 11809-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11809-1-AP (PSG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PSG1 Polyclonal antibody (11809-1-AP)

    IHC staining of human placenta using 11809-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11809-1-AP (PSG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PSG1 Polyclonal antibody (11809-1-AP)

    IF Staining of MCF-7 using 11809-1-AP

    Immunofluorescent analysis of MCF-7 cells using 11809-1-AP (PSG1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman kidney tissue, MCF-7 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

11809-1-AP targets PSG1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSG1 fusion protein Ag2468
Full Name pregnancy specific beta-1-glycoprotein 1
Calculated Molecular Weight 426 aa, 48 kDa
Observed Molecular Weight 72 kDa, 36 kDa
GenBank Accession NumberBC022338
Gene Symbol PSG1
Gene ID (NCBI) 5669
RRIDAB_2171045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PSG1 antibody 11809-1-APDownload protocol
IHC protocol for PSG1 antibody 11809-1-APDownload protocol
IF protocol for PSG1 antibody 11809-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Fertil Steril

Trophoblast retrieval and isolation from the cervix (TRIC) for noninvasive prenatal screening at 5 to 20 weeks of gestation.

Authors - Jay M Bolnick
View Article