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PSMD4 Monoclonal antibody

PSMD4 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3G1A1

Cat no : 66179-1-Ig

Synonyms

AF, AF 1, Antisecretory factor 1, ASF, MCB1, PSMD4, pUB R5, Rpn10, S5A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMD4 Monoclonal antibody (66179-1-Ig)

    WB analysis using 66179-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66179-1-Ig (PSMD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of human testis tissue using PSMD4 Monoclonal antibody (66179-1-Ig)

    IHC staining of human testis using 66179-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66179-1-Ig (PSMD4 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PSMD4 Monoclonal antibody (66179-1-Ig)

    IHC staining of human testis using 66179-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66179-1-Ig (PSMD4 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMD4 Monoclonal antibody (66179-1-Ig)

    IF Staining of HeLa using 66179-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMD4 antibody (66179-1-Ig, Clone: 3G1A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inU2OS cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66179-1-Ig targets PSMD4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSMD4 fusion protein Ag7010
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC002365
Gene Symbol PSMD4
Gene ID (NCBI) 5710
RRIDAB_2881574
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.

Protocols

Product Specific Protocols
WB protocol for PSMD4 antibody 66179-1-IgDownload protocol
IHC protocol for PSMD4 antibody 66179-1-IgDownload protocol
IF protocol for PSMD4 antibody 66179-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols