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PTH1R Polyclonal antibody

PTH1R Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24275-1-AP

Synonyms

parathyroid hormone 1 receptor, PFE, PTH/PTHr receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTH1R, PTHR, PTHR1

Validation Data Gallery

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  • Western Blot (WB) analysis of U2OS cells using PTH1R Polyclonal antibody (24275-1-AP)

    WB analysis of U2OS using 24275-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 24275-1-AP (PTH1R antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PTH1R Polyclonal antibody (24275-1-AP)

    WB analysis using 24275-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24275-1-AP (PTH1R antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PTH1R Polyclonal antibody (24275-1-AP)

    IHC staining of mouse kidney using 24275-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24275-1-AP (PTH1R antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PTH1R Polyclonal antibody (24275-1-AP)

    IHC staining of mouse kidney using 24275-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24275-1-AP (PTH1R antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PTH1R Polyclonal antibody (24275-1-AP)

    IHC staining of human pancreas cancer using 24275-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24275-1-AP (PTH1R antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PTH1R Polyclonal antibody (24275-1-AP)

    IHC staining of human pancreas cancer using 24275-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24275-1-AP (PTH1R antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inU2OS cells, HepG2 cells, mouse liver tissue
Positive IHC detected inmouse kidney tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24275-1-AP targets PTH1R in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTH1R fusion protein Ag17577
Full Name parathyroid hormone 1 receptor
Calculated Molecular Weight 593 aa, 66 kDa
Observed Molecular Weight 70-80 kDa
GenBank Accession NumberBC110388
Gene Symbol PTH1R
Gene ID (NCBI) 5745
RRIDAB_2879465
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTH1R is a receptor for parathyroid hormone and parathyroid hormone-related peptide (PTHrP). PTH1R is a glycoprotein with seven transmembrane domains and it has an extracellular N-terminus region and an intracellular C-terminus region. The activity of PTH1R is mediated by G proteins which activate adenylyl cyclase. It has been reported that PTHrP and PTH1R are associated with the differentiation of bone and cartilage in the developing stages. PTH1R has several isoforms with 66/59 kDa and PTH1R can form a 120 kDa dimer in the non-reducing condition. 24275-1-AP antibody detects 80 kDa protein which is similar to the results reported in publications.(PMID: 19507249, 27160269)

Protocols

Product Specific Protocols
WB protocol for PTH1R antibody 24275-1-APDownload protocol
IHC protocol for PTH1R antibody 24275-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols