• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PTPN18 Polyclonal antibody

PTPN18 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17551-1-AP

Synonyms

BDP1, Brain derived phosphatase, PTP HSCF, PTPN18

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Jurkat cells using PTPN18 Polyclonal antibody (17551-1-AP)

    WB analysis of Jurkat using 17551-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17551-1-AP (PTPN18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PTPN18 Polyclonal antibody (17551-1-AP)

    WB analysis of human brain using 17551-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17551-1-AP (PTPN18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using PTPN18 Polyclonal antibody (17551-1-AP)

    IHC staining of human brain using 17551-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17551-1-AP (PTPN18 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using PTPN18 Polyclonal antibody (17551-1-AP)

    IHC staining of human brain using 17551-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17551-1-AP (PTPN18 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inJurkat cells, human brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17551-1-AP targets PTPN18 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPN18 fusion protein Ag11704
Full Name protein tyrosine phosphatase, non-receptor type 18 (brain-derived)
Calculated Molecular Weight 460 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC052800
Gene Symbol PTPN18
Gene ID (NCBI) 26469
RRIDAB_2284599
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPN18(Tyrosine-protein phosphatase non-receptor type 18) is also named as BDP1 and belongs to the protein-tyrosine phosphatase family. The PTPN18 cDNA encodes a deduced 459-amino acid protein with no transmembrane domain and a calculated molecular weight of 50 kD. PTPN18 has a PEST motif and shares the greatest homology with members of the PTP-PEST gene family. Northern blot analysis indicated that PTPN18 is expressed not only in brain, but also in colon and several different tumor-derived cell lines.(PMID:8950995). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PTPN18 antibody 17551-1-APDownload protocol
IHC protocol for PTPN18 antibody 17551-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cell Physiol Biochem

Prognostic Value of Phosphotyrosine Phosphatases in Hepatocellular Carcinoma.

Authors - Guangyan Zhangyuan
View Article
humanWB

Clin Exp Pharmacol Physiol

Downregulation of PTPN18 can inhibit proliferation and metastasis and promote apoptosis of endometrial cancer.

Authors - Junhong Cai
View Article