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PTPN9 Polyclonal antibody

PTPN9 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12109-1-AP

Synonyms

MEG2, PTPase MEG2, PTPMEG2, PTPN9

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using PTPN9 Polyclonal antibody (12109-1-AP)

    WB analysis of mouse pancreas using 12109-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of A549 cells using PTPN9 Polyclonal antibody (12109-1-AP)

    WB analysis of A549 using 12109-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PTPN9 Polyclonal antibody (12109-1-AP)

    WB analysis of mouse liver using 12109-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PTPN9 Polyclonal antibody (12109-1-AP)

    WB analysis using 12109-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using PTPN9 Polyclonal antibody (12109-1-AP)

    WB analysis of Transfected HEK-293 using 12109-1-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using PTPN9 Polyclonal antibody (12109-1-AP)

    WB analysis of Transfected HEK-293 using 12109-1-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using PTPN9 Polyclonal antibody (12109-1-AP)

    IP experiment of HEK-293 using 12109-1-AP

    IP result of anti-PTPN9 (IP:12109-1-AP, 4ug; Detection:12109-1-AP 1:300) with HEK-293 cells lysate 1800ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PTPN9 Polyclonal antibody (12109-1-AP)

    IHC staining of human liver cancer using 12109-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12109-1-AP (PTPN9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PTPN9 Polyclonal antibody (12109-1-AP)

    IHC staining of human liver cancer using 12109-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12109-1-AP (PTPN9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, Transfected HEK-293 cells, mouse liver tissue, mouse pancreas tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12109-1-AP targets PTPN9 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPN9 fusion protein Ag2754
Full Name protein tyrosine phosphatase, non-receptor type 9
Calculated Molecular Weight 593 aa, 68 kDa
Observed Molecular Weight 68-70 kDa
GenBank Accession NumberBC010863
Gene Symbol PTPN9
Gene ID (NCBI) 5780
RRIDAB_2877826
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPN9, Tyrosine-protein phosphatase non-receptor type 9, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN9 is involved in the transfer of hydrophobic ligands or in functions of the Golgi apparatus (PMID: 19167335). MiR-96 inhibits PTPN9 expression and consequently promotes proliferation, migration and invasion of breast cancer cells (PMID:27857177). PTPN9 can be detected double bands (PMID: 22394684). PTPN9 was located in cytoplasm and nucleus in human breast cancer (PMID: 23418360).

Protocols

Product Specific Protocols
WB protocol for PTPN9 antibody 12109-1-APDownload protocol
IHC protocol for PTPN9 antibody 12109-1-APDownload protocol
IP protocol for PTPN9 antibody 12109-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Ther Nucleic Acids

MicroRNA-126 Regulates Angiogenesis and Neurogenesis in a Mouse Model of Focal Cerebral Ischemia.

Authors - Meijie Qu
View Article