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PTPRCAP / CD45AP Polyclonal antibody

PTPRCAP / CD45AP Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19715-1-AP

Synonyms

CD45 AP, CD45 associated protein, LPAP, PTPRC associated protein, PTPRCAP, PTPRCAP / CD45AP

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using PTPRCAP / CD45AP Polyclonal antibody (19715-1-AP)

    WB analysis of Jurkat using 19715-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PTPRCAP / CD45AP Polyclonal antibody (19715-1-AP)

    WB analysis of Jurkat using 19715-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using PTPRCAP / CD45AP Polyclonal antibody (19715-1-AP)

    WB analysis of human spleen using 19715-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PTPRCAP / CD45AP Polyclonal antibody (19715-1-AP)

    IHC staining of human tonsillitis using 19715-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PTPRCAP / CD45AP Polyclonal antibody (19715-1-AP)

    IHC staining of human tonsillitis using 19715-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PTPRCAP / CD45AP Polyclonal antibody (19715-1-AP)

    IHC staining of human tonsillitis using 19715-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using PTPRCAP / CD45AP Polyclonal antibody (19715-1-AP)

    IHC staining of human lymphoma using 19715-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using PTPRCAP / CD45AP Polyclonal antibody (19715-1-AP)

    IHC staining of human lymphoma using 19715-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using PTPRCAP / CD45AP Polyclonal antibody (19715-1-AP)

    IF Staining of Jurkat using 19715-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PTPRCAP / CD45AP antibody (19715-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, human spleen tissue
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19715-1-AP targets PTPRCAP / CD45AP in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name protein tyrosine phosphatase, receptor type, C-associated protein
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberNM_005608
Gene Symbol PTPRCAP
Gene ID (NCBI) 5790
RRIDAB_10860107
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPRCAP, also named as LPAP and CD45-AP, interacts with CD45/PTPRC.

Protocols

Product Specific Protocols
WB protocol for PTPRCAP / CD45AP antibody 19715-1-APDownload protocol
IHC protocol for PTPRCAP / CD45AP antibody 19715-1-APDownload protocol
IF protocol for PTPRCAP / CD45AP antibody 19715-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols