IA-2/PTPRN Polyclonal antibody
IA-2/PTPRN Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 10584-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, HEK-293 cells, mouse kidney tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse pancreas tissue |
| Positive FC detected in | HEK-293 cells, Y79 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 2 publications below |
Product Information
10584-1-AP targets IA-2/PTPRN in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IA-2/PTPRN fusion protein Ag0923 |
| Full Name | protein tyrosine phosphatase, receptor type, N |
| Calculated Molecular Weight | 105 kDa |
| Observed Molecular Weight | 60-65 kDa, 106-115 kDa |
| GenBank Accession Number | BC007713 |
| Gene Symbol | PTPRN |
| Gene ID (NCBI) | 5798 |
| RRID | AB_2237983 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell).(PMID: 10027571). IA-2/PTPRN was found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IA-2/PTPRN antibody 10584-1-AP | Download protocol |
| IHC protocol for IA-2/PTPRN antibody 10584-1-AP | Download protocol |
| IF protocol for IA-2/PTPRN antibody 10584-1-AP | Download protocol |
| IP protocol for IA-2/PTPRN antibody 10584-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Overexpression of PTPRN Promotes Metastasis of Lung Adenocarcinoma and Suppresses NK Cell Cytotoxicity. | ||
J Biol Chem Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress. | ||
PLoS One Sensitive detection of multiple islet autoantibodies in type 1 diabetes using small sample volumes by agglutination-PCR. | ||
Cell Physiol Biochem Prognostic Value of Phosphotyrosine Phosphatases in Hepatocellular Carcinoma. |
