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  • Featured Product
  • KD/KO Validated

PYGO2 Polyclonal antibody

PYGO2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11555-1-AP

Synonyms

1190004M21Rik, PYGO2, Pygopus homolog 2, pygopus homolog 2 (Drosophila)

Validation Data Gallery

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  • Western Blot (WB) analysis of MDA-MB-453s cells using PYGO2 Polyclonal antibody (11555-1-AP)

    WB analysis of MDA-MB-453s using 11555-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using PYGO2 Polyclonal antibody (11555-1-AP)

    WB analysis of C6 using 11555-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using PYGO2 Polyclonal antibody (11555-1-AP)

    WB analysis of C6 using 11555-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using PYGO2 Polyclonal antibody (11555-1-AP)

    WB analysis of MDA-MB-453s using 11555-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PYGO2 Polyclonal antibody (11555-1-AP)

    WB analysis of HeLa using 11555-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PYGO2 Polyclonal antibody (11555-1-AP)

    WB analysis of HeLa using 11555-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMDA-MB-453s cells, C6 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11555-1-AP targets PYGO2 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PYGO2 fusion protein Ag2111
Full Name pygopus homolog 2 (Drosophila)
Calculated Molecular Weight 406 aa, 41 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC032099
Gene Symbol PYGO2
Gene ID (NCBI) 90780
RRIDAB_2877776
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYGO2, also named as Pygopus homolog 2, is a 406 animo acid protein, which contains 1 PHD-type zinc finger. PYGO2 is involved in signal transduction through the Wnt pathway and Binds to BCL9 via the PHD-type zinc finger motif, and thereby becomes part of the nuclear beta-catenin/TCF complex. PYGO2 is in a high proportion of breast and epithelial ovarian malignant tumors and is required for the growth of several cell lines derived from these carcinomas.

Protocols

Product Specific Protocols
WB protocol for PYGO2 antibody 11555-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Cancer

Pygo2 as a novel biomarker in gastric cancer for monitoring drug resistance by upregulating MDR1.

Authors - Dongdong Zhang
  • KD Validated
View Article
humanWB

Nat Commun

Structural basis of the interaction between BCL9-Pygo and LDB-SSBP complexes in assembling the Wnt enhanceosome

Authors - Hongyang Wang
View Article