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Phospho-GSK3A (Ser21) Polyclonal antibody

Phospho-GSK3A (Ser21) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

Conjugate

Unconjugated

Cat no : 28875-1-AP

Synonyms

DKFZp686D0638, GSK 3 alpha, GSK3A, Phospho-GSK3A (Ser21)

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-GSK3A (Ser21) Polyclonal antibody (28875-1-AP)

    WB analysis using 28875-1-AP

    Non-treated PC-3 and Calyculin A treated treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28875-1-AP (Phospho-GSK3A (Ser21) antibody) at dilution of 1:3000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Tested Applications

Positive WB detected inCalyculin A treated PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28875-1-AP targets Phospho-GSK3A (Ser21) in WB applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name glycogen synthase kinase 3 alpha
Calculated Molecular Weight 483 aa, 51 kDa
Observed Molecular Weight51 kDa
GenBank Accession NumberBC027984
Gene Symbol GSK3A
Gene ID (NCBI) 2931
RRIDAB_2918211
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSK3A (glycogen synthase kinase 3 alpha), one of the two functional isoforms encoded by the GSK3 gene, is a serine/threonine protein kinase that is involved in cell-cycle progression, differentiation, and apoptosis (PMID: 27049759). Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin.GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3A and Ser9 of GSK-3B. GSK3A acts as a tumor suppressor and has important roles in acute myeloid leukemia (AML), prostate cancer, and pancreatic cancer (PMID: 22326953, 22539113, 23547054).

Protocols

Product Specific Protocols
WB protocol for Phospho-GSK3A (Ser21) antibody 28875-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols