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  • Featured Product
  • KD/KO Validated

RAB11FIP2 Polyclonal antibody

RAB11FIP2 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 18136-1-AP

Synonyms

KIAA0941, nRip11, Rab11 FIP2, RAB11FIP2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB11FIP2 Polyclonal antibody (18136-1-AP)

    WB analysis using 18136-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18136-1-AP (RAB11FIP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using RAB11FIP2 Polyclonal antibody (18136-1-AP)

    WB analysis of mouse liver using 18136-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18136-1-AP (RAB11FIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RAB11FIP2 Polyclonal antibody (18136-1-AP)

    WB analysis of HEK-293 using 18136-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18136-1-AP (RAB11FIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAB11FIP2 Polyclonal antibody (18136-1-AP)

    IF Staining of HeLa using 18136-1-AP

    Immunofluorescent analysis of Hela cells, using RAB11FIP2 antibody 18136-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RAB11FIP2 Polyclonal antibody (18136-1-AP)

    IF Staining of HepG2 using 18136-1-AP

    Immunofluorescent analysis of HepG2 cells, using RAB11FIP2 antibody 18136-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inmouse brain tissue, mouse liver tissue, HEK-293 cells, rat brain tissue
Positive IF detected inHela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18136-1-AP targets RAB11FIP2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB11FIP2 fusion protein Ag12736
Full Name RAB11 family interacting protein 2 (class I)
Calculated Molecular Weight 512 aa, 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC075073
Gene Symbol RAB11FIP2
Gene ID (NCBI) 22841
RRIDAB_10597110
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RAB11FIP2 antibody 18136-1-APDownload protocol
IF protocol for RAB11FIP2 antibody 18136-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

PLoS Pathog

Acquisition of Rab11 and Rab11-Fip2-A novel strategy for Chlamydia pneumoniae early survival.

Authors - Katja Mölleken
  • KD Validated
View Article
humanWB

Traffic

Rab11-FIP2 Interaction with MYO5B Regulates Movement of Rab11a-Containing Recycling Vesicles.

Authors - Jenny C Schafer
View Article
humanIF

Exp Cell Res

Rab11-FIP1A regulates early trafficking into the recycling endosomes.

Authors - Jenny C Schafer
View Article