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RAB3GAP2 Monoclonal antibody

RAB3GAP2 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

3A4A1

Cat no : 66648-1-Ig

Synonyms

DKFZp434D245, FLJ14579, KIAA0839, p150, Rab3 GAP p150, Rab3 GAP regulatory subunit, RAB3 GAP150, RAB3GAP150, RAB3GAP2, RGAP iso

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB3GAP2 Monoclonal antibody (66648-1-Ig)

    WB analysis using 66648-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66648-1-Ig (RAB3GAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66648-1-Ig targets RAB3GAP2 in WB,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RAB3GAP2 fusion protein Ag20254
Full Name RAB3 GTPase activating protein subunit 2 (non-catalytic)
Calculated Molecular Weight 1393 aa, 156 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC146760
Gene Symbol RAB3GAP2
Gene ID (NCBI) 25782
RRIDAB_2882005
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB3GAP2, also known as RAB3-GAP150, belongs to the Rab3-GAP regulatory subunit family. Rab3 GAP consists of two subunits: the catalytic subunit p130 and the noncatalytic subunit p150. Rab3 GAP is ubiquitously expressed and enriched in the synaptic soluble fraction of brain, consistent with it having a key role in neurodevelopment. RAB3-GAP150 forms the Rab3 GTPase-activating complex with RAB3-GAP130,where it constitutes the regulatory subunit, whereas the latter functions as the catalytic subunit. Defects in RAB3-GAP150 are the cause of Martsolf and Warburg Micro syndrome.

Protocols

Product Specific Protocols
WB protocol for RAB3GAP2 antibody 66648-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols