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CoraLite® Plus 488-conjugated RACGAP1 Monoclonal antibody

RACGAP1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6E10A2

Cat no : CL488-66056

Synonyms

HsCYK 4, ID GAP, KIAA1478, Male germ cell RacGap, MgcRacGAP, RACGAP1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated RACGAP1 Monoclonal a (CL488-66056)

    IF Staining of HeLa using CL488-66056

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL488-66056 (RACGAP1 antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-66056 is RACGAP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RACGAP1 fusion protein Ag6944
Full Name Rac GTPase activating protein 1
Calculated Molecular Weight 632 aa, 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC032754
Gene Symbol RACGAP1
Gene ID (NCBI) 29127
RRIDAB_2883243
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rac GTPase-activating protein 1 (RACGAP1), localized in the mitotic spindle, has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis, control of cell growth and differentiation, regulation of spermatogenesis, and tumor metastasis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RACGAP1 antibody CL488-66056Download protocol
Standard Protocols
Click here to view our Standard Protocols