- Featured Product
- KD/KO Validated
RAD51AP1 Polyclonal antibody
RAD51AP1 Polyclonal Antibody for WB, IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 11255-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, human brain tissue, mouse liver tissue, rat liver tissue, Raji cells |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 8 publications below |
| IF | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
11255-1-AP targets RAD51AP1 in WB, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RAD51AP1 fusion protein Ag1781 |
| Full Name | RAD51 associated protein 1 |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | BC016330 |
| Gene Symbol | RAD51AP1 |
| Gene ID (NCBI) | 10635 |
| RRID | AB_2300786 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Homologous recombination (HR) is an important pathway for the elimination of DNA double-strand breaks (DSBs) and other deleterious lesions from chromosomes. The eukaryotic HR reaction is mediated by RAD51 [PMID:12456786]. RAD51AP1 (RAD51 associated protein 1), so named because it can interact with human RAD51, is a vertebrate-specific protein that enhances the ability of RAD51 to mediate the D-loop reaction. RAD51AP1 knockdown by siRNA leads to cellular sensitivity to DNA damaging agents and elevated DNA damage-induced chromatid breaks. Enhancement of the RAD51 recombinase activity by RAD51AP1 is contingent upon physical interaction of the two HR factors [PMID:17996710,17996711].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAD51AP1 antibody 11255-1-AP | Download protocol |
| IF protocol for RAD51AP1 antibody 11255-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature RNA transcripts stimulate homologous recombination by forming DR-loops.
| ||
Mol Cell TERRA and RAD51AP1 promote alternative lengthening of telomeres through an R- to D-loop switch | ||
Oncogene BRD4 inhibition sensitizes cervical cancer to radiotherapy by attenuating DNA repair. | ||
Front Genet Systematic pan-cancer analysis showed that RAD51AP1 was associated with immune microenvironment, tumor stemness, and prognosis | ||
J Cell Biochem The transcriptional regulator Zfat is essential for maintenance and differentiation of the adipocytes. | ||
Int J Oncol METTL3 antagonizes 5‑FU chemotherapy and confers drug resistance in colorectal carcinoma. |
