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RALYL Polyclonal antibody

RALYL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17179-1-AP

Synonyms

hnRNP core protein C like 3, HNRPCL3, hRALYL, RALY RNA binding protein like, RALYL, RNA binding Raly like protein

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human brain tissue using RALYL Polyclonal antibody (17179-1-AP)

    WB analysis of human brain using 17179-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17179-1-AP (RALYL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RALYL Polyclonal antibody (17179-1-AP)

    IHC staining of mouse brain using 17179-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17179-1-AP (RALYL Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RALYL Polyclonal antibody (17179-1-AP)

    IHC staining of mouse brain using 17179-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17179-1-AP (RALYL Antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 17179-1-AP is RALYL Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RALYL fusion protein Ag10898
Full Name RALY RNA binding protein-like
Calculated Molecular Weight 291 aa, 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC031090
Gene Symbol RALYL
Gene ID (NCBI) 138046
RRIDAB_2878355
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RALYL antibody 17179-1-APDownload protocol
IHC protocol for RALYL antibody 17179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

Long noncoding RNA AFAP1-AS1 promotes tumor progression and invasion by regulating the miR-2110/Sp1 axis in triple-negative breast cancer.

Authors - Xiaohui Zhang
View Article