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RALYL Monoclonal antibody

RALYL Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1F3F4

Cat no : 67534-1-Ig

Synonyms

hnRNP core protein C like 3, HNRPCL3, hRALYL, RALY RNA binding protein like, RALYL, RNA binding Raly like protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RALYL Monoclonal antibody (67534-1-Ig)

    WB analysis using 67534-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67534-1-Ig (RALYL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RALYL Monoclonal antibody (67534-1-Ig)

    WB analysis using 67534-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67534-1-Ig (RALYL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using RALYL Monoclonal antibody (67534-1-Ig)

    WB analysis of pig brain using 67534-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67534-1-Ig (RALYL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using RALYL Monoclonal antibody (67534-1-Ig)

    WB analysis of rat brain using 67534-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67534-1-Ig (RALYL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using RALYL Monoclonal antibody (67534-1-Ig)

    WB analysis of mouse brain using 67534-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67534-1-Ig (RALYL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RALYL Monoclonal antibody (67534-1-Ig)

    IF Staining of HepG2 using 67534-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67534-1-Ig (RALYL antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, pig brain tissue, rat brain tissue, mouse brain tissue, HEK-293 cells, HepG2 cells, Raji cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67534-1-Ig targets RALYL in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RALYL fusion protein Ag11676
Full Name RALY RNA binding protein-like
Calculated Molecular Weight 291 aa, 32 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC031090
Gene Symbol RALYL
Gene ID (NCBI) 138046
RRIDAB_2882753
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RALYL antibody 67534-1-IgDownload protocol
IF protocol for RALYL antibody 67534-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols