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  • KD/KO Validated

RASGRP3 Polyclonal antibody

RASGRP3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13162-1-AP

Synonyms

GRP3, KIAA0846, Ras guanyl releasing protein 3, RAS-GRP, RASGRP3



Tested Applications

Positive WB detected inRAW 264.7 cells, PC-3 cells, Raji cells, Ramos cells
Positive IP detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13162-1-AP targets RASGRP3 in WB, IP, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RASGRP3 fusion protein Ag3810
Full Name RAS guanyl releasing protein 3 (calcium and DAG-regulated)
Calculated Molecular Weight 690 aa, 78 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC027849
Gene Symbol RASGRP3
Gene ID (NCBI) 25780
RRIDAB_10640522
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AS guanyl releasing protein 3 (RasGRP3) is a member of the RasGRP family and is able to promote guanine nucleotide exchange of Ha-Ras, R-Ras and Rap1 (PMID: 9582122). RasGRP3 is involved in a diverse range of important biological processes. RasGRP3 has emerged as an important mediator of signaling downstream from receptor coupled phosphoinositide turnover in B and T cells. Overexpression of RasGRP3 is commonly associated with many malignancies, such as pre-Bcell leukemia, Burkitt's lymphoma and natural killer-like T-cell leukemia. Recently, it has been reported that upregulation of RASGRP3 expression in prostate cancer correlates with aggressive capabilities and predicts biochemical recurrence after radical prostatectomy (PMID: 24418912).

Protocols

Product Specific Protocols
WB protocol for RASGRP3 antibody 13162-1-APDownload protocol
IHC protocol for RASGRP3 antibody 13162-1-APDownload protocol
IP protocol for RASGRP3 antibody 13162-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancer Cell

RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma.

Authors - Xu Chen
humanWB,FC

BMC Med

Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis

Authors - Zhijian Zheng
  • KD Validated
humanWB,IHC

Am J Cancer Res

A somatic mutation of RasGRP3 decreases Na+/I- symporter expression in metastases of radioactive iodine-refractory thyroid cancer by stimulating the Akt signaling pathway.

Authors - Jianlu Song
humanWB,IHC

Gene

RasGRP3 controls cell proliferation and migration in papillary thyroid cancer by regulating the Akt-MDM2 pathway.

Authors - Wangwang Qiu
  • KD Validated
humanWB, IHC

J Vet Sci

Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus.

Authors - Lishan Lv
humanWB,IHC

Prostate Cancer Prostatic Dis

Upregulation of RASGRP3 expression in prostate cancer correlates with aggressive capabilities and predicts biochemical recurrence after radical prostatectomy.

Authors - X Zeng