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PAMCI Polyclonal antibody

PAMCI Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12861-1-AP

Synonyms

P CIP1, PAMCI, PCIP1, RASSF9

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using PAMCI Polyclonal antibody (12861-1-AP)

    WB analysis of HepG2 using 12861-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12861-1-AP (PAMCI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PAMCI Polyclonal antibody (12861-1-AP)

    WB analysis using 12861-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12861-1-AP (PAMCI antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using PAMCI Polyclonal antibody (12861-1-AP)

    WB analysis of human liver using 12861-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12861-1-AP (PAMCI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHepG2 cells, human liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12861-1-AP targets PAMCI in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAMCI fusion protein Ag3976
Full Name Ras association (RalGDS/AF-6) domain family (N-terminal) member 9
Calculated Molecular Weight 435 aa, 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC031589
Gene Symbol RASSF9
Gene ID (NCBI) 9182
RRIDAB_2238175
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PAMCI antibody 12861-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols