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RBM46 Polyclonal antibody

RBM46 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24368-1-AP

Synonyms

Cancer/testis antigen 68, CT68, RBM46, RNA binding motif protein 46

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using RBM46 Polyclonal antibody (24368-1-AP)

    WB analysis of Jurkat using 24368-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24368-1-AP (RBM46 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RBM46 Polyclonal antibody (24368-1-AP)

    WB analysis of Jurkat using 24368-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24368-1-AP (RBM46 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using RBM46 Polyclonal antibody (24368-1-AP)

    IHC staining of human kidney using 24368-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24368-1-AP (RBM46 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using RBM46 Polyclonal antibody (24368-1-AP)

    IHC staining of human kidney using 24368-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24368-1-AP (RBM46 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24368-1-AP targets RBM46 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM46 fusion protein Ag21653
Full Name RNA binding motif protein 46
Calculated Molecular Weight 533 aa, 60 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC028588
Gene Symbol RBM46
Gene ID (NCBI) 166863
RRIDAB_2879509
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RBM46 antibody 24368-1-APDownload protocol
IHC protocol for RBM46 antibody 24368-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols