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RDH5 Polyclonal antibody

RDH5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13140-1-AP

Synonyms

11 cis RDH, 11 cis retinol dehydrogenase, HSD17B9, RDH1, RDH5, SDR9C5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RDH5 Polyclonal antibody (13140-1-AP)

    WB analysis using 13140-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13140-1-AP (RDH5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse eye tissue, rat eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13140-1-AP targets RDH5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RDH5 fusion protein Ag3784
Full Name retinol dehydrogenase 5 (11-cis/9-cis)
Calculated Molecular Weight 318 aa, 35 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC028298
Gene Symbol RDH5
Gene ID (NCBI) 5959
RRIDAB_2918031
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RDH5, also named as RDH1, SDR9C5 and HSD17B9, belongs to short-chain dehydrogenases/reductases (SDR) family. RDH5 catalyzes the oxidation of cis-isomers of retinol in an NAD-dependent manner for the final step in the biosynthesis of 11-cis retinaldehyde. Mutations in this gene are associated with fundus albipunctatus, an autosomal recessive eye disease characterized by stationary night blindness and accumulation of white spots in the retina (PubMed:11675386). The predicted size of this protein is 35 kDa. Some paper have reported that the molecular weight is 32 kDa and could form a dimer of about 60 kDa.

Protocols

Product Specific Protocols
WB protocol for RDH5 antibody 13140-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols