• Featured Product
  • KD/KO Validated

RGMA Polyclonal antibody

RGMA Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12387-1-AP

Synonyms

Repulsive guidance molecule A, RGM, RGM domain family member A, RGM domain family, member A, RGMA



Tested Applications

Positive WB detected inCOLO 320 cells, mouse testis tissue, HeLa cells, MOLT-4 cells, U2OS cells, Caco-2 cells, K-562 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12387-1-AP targets RGMA in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RGMA fusion protein Ag3058
Full Name RGM domain family, member A
Calculated Molecular Weight 450 aa, 49 kDa
Observed Molecular Weight49 kDa
GenBank Accession NumberBC015886
Gene Symbol RGMA
Gene ID (NCBI) 56963
RRIDAB_2179366
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RGMA, also named as RGM, is a member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. It regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. RGMA binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS1-PTK2-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8. RGMA is a molecular target for neuroprotection in retinal pathologies(PMID: 20457227). the frequent genetic and epigenetic inactivation of RGMA in CRCs and adenomas along with its in vitro function collectively support its role as a tumor suppressor in colon cells(PMID:19303019 ). There're some different MW in WB detection, 30 kDa mature form, 47-50 kDa isoforms and 60 kDa Glycosylated form.

Protocols

Product Specific Protocols
WB protocol for RGMA antibody 12387-1-APDownload protocol
IHC protocol for RGMA antibody 12387-1-APDownload protocol
IP protocol for RGMA antibody 12387-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Brain Res Bull

Adenoviral Vector-Induced Silencing of RGMa Attenuates Blood-Brain Barrier Dysfunction in a Rat Model of MCAO/Reperfusion.

Authors - Min Li
  • KD Validated
mouseCoIP

Sci Adv

RGMa collapses the neuronal actin barrier against disease-implicated protein and exacerbates ALS

Authors - Mikito Shimizu