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RGR Polyclonal antibody

RGR Polyclonal Antibody for WB, IP, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11904-1-AP

Synonyms

RGR

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using RGR Polyclonal antibody (11904-1-AP)

    WB analysis of human heart using 11904-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11904-1-AP (RGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of PC-3 cells using RGR Polyclonal antibody (11904-1-AP)

    IP experiment of PC-3 using 11904-1-AP

    IP result of anti-RGR (IP:11904-1-AP, 4ug; Detection:11904-1-AP 1:300) with PC-3 cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of mouse retina tissue using RGR Polyclonal antibody (11904-1-AP)

    IHC staining of mouse retina using 11904-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse retina tissue slide using 11904-1-AP (RGR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse retina tissue using RGR Polyclonal antibody (11904-1-AP)

    IHC staining of mouse retina using 11904-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse retina tissue slide using 11904-1-AP (RGR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RGR Polyclonal antibody (11904-1-AP)

    IHC staining of human prostate cancer using 11904-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11904-1-AP (RGR antibody) at dilution of 1:100 (under 10x lens).

  • Flow cytometry (FC) experiment of PC-3 cells using RGR Polyclonal antibody (11904-1-AP)

    FC experiment of PC-3 using 11904-1-AP

    1X10^6 PC-3 cells were stained with 0.2ug RGR antibody (11904-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inhuman heart tissue
Positive IP detected inPC-3 cells
Positive IHC detected inmouse retina tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11904-1-AP targets RGR in WB, IP, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RGR fusion protein Ag2552
Full Name retinal G protein coupled receptor
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa, 26 kDa
GenBank Accession NumberBC008094
Gene Symbol RGR
Gene ID (NCBI) 5995
RRIDAB_2179498
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RGR is a G protein-coupled receptor preferentially expressed at high levels in the retinal pigment epithelium (RPE) and Mueller cells of the neural retina. Like other opsins which bind retinaldehyde, it contains a conserved lysine residue in the seventh transmembrane domain. RGR acts as a photoisomerase to catalyze the conversion of all-trans-retinal to 11-cis-retinal. The reverse isomerization occurs with rhodopsin in retinal photoreceptor cells. The gene of PGR may be associated with autosomal recessive and autosomal dominant retinitis pigmentosa (arRP and adRP, respectively). Alternative splicing results in multiple transcript variants encoding different isoforms.

Protocols

Product Specific Protocols
WB protocol for RGR antibody 11904-1-APDownload protocol
IHC protocol for RGR antibody 11904-1-APDownload protocol
IP protocol for RGR antibody 11904-1-APDownload protocol
FC protocol for RGR antibody 11904-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols