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RHAG Monoclonal antibody

RHAG Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1C1C4

Cat no : 67714-1-Ig

Synonyms

Ammonium transporter Rh type A, CD241, Rh associated glycoprotein, Rh family type A glycoprotein, Rh type A glycoprotein, RH2, Rh50, RH50A, Rh50GP, RHAG, SLC42A1

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using RHAG Monoclonal antibody (67714-1-Ig)

    WB analysis of human placenta using 67714-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67714-1-Ig (RHAG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using RHAG Monoclonal antibody (67714-1-Ig)

    IHC staining of human placenta using 67714-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67714-1-Ig (RHAG antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Immunohistochemistry (IHC) staining of human placenta tissue using RHAG Monoclonal antibody (67714-1-Ig)

    IHC staining of human placenta using 67714-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67714-1-Ig (RHAG antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Immunohistochemistry (IHC) staining of human spleen tissue using RHAG Monoclonal antibody (67714-1-Ig)

    IHC staining of human spleen using 67714-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67714-1-Ig (RHAG antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using RHAG Monoclonal antibody (67714-1-Ig)

    IHC staining of human spleen using 67714-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67714-1-Ig (RHAG antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using RHAG Monoclonal antibody (67714-1-Ig)

    IF Staining of human placenta using 67714-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using RHAG antibody (67714-1-Ig, Clone: 1C1C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using RHAG Monoclonal antibody (67714-1-Ig)

    IF Staining of human placenta using 67714-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using RHAG antibody (67714-1-Ig, Clone: 1C1C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67714-1-Ig targets RHAG in WB, IHC, IF,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RHAG fusion protein Ag30130
Full Name Rh-associated glycoprotein
Calculated Molecular Weight 409 aa, 44 kDa
Observed Molecular Weight50-60 kDa
GenBank Accession NumberBC012605
Gene Symbol RHAG
Gene ID (NCBI) 6005
RRIDAB_2882904
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RHAG antibody 67714-1-IgDownload protocol
IHC protocol for RHAG antibody 67714-1-IgDownload protocol
IF protocol for RHAG antibody 67714-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols