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RPL19 Polyclonal antibody

RPL19 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14701-1-AP

Synonyms

60S ribosomal protein L19, ribosomal protein L19, RPL19

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RPL19 Polyclonal antibody (14701-1-AP)

    WB analysis of HeLa using 14701-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14701-1-AP (RPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using RPL19 Polyclonal antibody (14701-1-AP)

    WB analysis of PC-3 using 14701-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14701-1-AP (RPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RPL19 Polyclonal antibody (14701-1-AP)

    WB analysis of MCF-7 using 14701-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14701-1-AP (RPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using RPL19 Polyclonal antibody (14701-1-AP)

    WB analysis of mouse testis using 14701-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14701-1-AP (RPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using RPL19 Polyclonal antibody (14701-1-AP)

    WB analysis of mouse spleen using 14701-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14701-1-AP (RPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RPL19 Polyclonal antibody (14701-1-AP)

    IP experiment of HeLa using 14701-1-AP

    IP result of anti-RPL19 (IP:14701-1-AP, 4ug; Detection:14701-1-AP 1:3000) with HeLa cells lysate 1560 ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using RPL19 Polyclonal antibody (14701-1-AP)

    IHC staining of human brain using 14701-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14701-1-AP (RPL19 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RPL19 Polyclonal antibody (14701-1-AP)

    IHC staining of human brain using 14701-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14701-1-AP (RPL19 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RPL19 Polyclonal antibody (14701-1-AP)

    IHC staining of mouse brain using 14701-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14701-1-AP (RPL19 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RPL19 Polyclonal antibody (14701-1-AP)

    IHC staining of mouse brain using 14701-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14701-1-AP (RPL19 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RPL19 Polyclonal antibody (14701-1-AP)

    IF Staining of HeLa using 14701-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14701-1-AP (RPL19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using RPL19 Polyclonal antibody (14701-1-AP)

    FC experiment of HeLa using 14701-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human RPL19 (14701-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, mouse spleen tissue, mouse testis tissue, PC-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14701-1-AP targets RPL19 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL19 fusion protein Ag6371
Full Name ribosomal protein L19
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC066315
Gene Symbol RPL19
Gene ID (NCBI) 6143
RRIDAB_2181587
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPL19, also named as 60S ribosomal protein L19, is a 196 amino acid protein, which belongs to the ribosomal protein L19e family. RPL19 encodes a ribosomal protein that is a component of the 60S subunit.

Protocols

Product Specific Protocols
WB protocol for RPL19 antibody 14701-1-APDownload protocol
IHC protocol for RPL19 antibody 14701-1-APDownload protocol
IF protocol for RPL19 antibody 14701-1-APDownload protocol
IP protocol for RPL19 antibody 14701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,FC

Transl Res

Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.

Authors - Mahmoud Dabbah
View Article
mouseWB

Dev Cell

Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.

Authors - Yuqing Lei
View Article
mouseWB

EMBO J

Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.

Authors - Harrison Tudor Evans
View Article
humanWB

Curr Biol

RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.

Authors - Long Jiang
View Article
humanWB

Hum Mutat

Frameshift mutation in p53 regulator RPL26 is associated with multiple physical abnormalities and a specific pre-ribosomal RNA processing defect in diamond-blackfan anemia.

Authors - Gazda Hanna T HT
View Article

EMBO J

Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.

Authors - Harrison Tudor Evans
View Article