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RPL38 Polyclonal antibody

RPL38 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15055-1-AP

Synonyms

60S ribosomal protein L38, ribosomal protein L38, RPL38

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RPL38 Polyclonal antibody (15055-1-AP)

    WB analysis of HeLa using 15055-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15055-1-AP (RPL38 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RPL38 Polyclonal antibody (15055-1-AP)

    WB analysis of MCF-7 using 15055-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15055-1-AP (RPL38 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RPL38 Polyclonal antibody (15055-1-AP)

    IHC staining of human pancreas cancer using 15055-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 15055-1-AP (RPL38 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RPL38 Polyclonal antibody (15055-1-AP)

    IHC staining of human pancreas cancer using 15055-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 15055-1-AP (RPL38 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPL38 Polyclonal antibody (15055-1-AP)

    IHC staining of human colon cancer using 15055-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15055-1-AP (RPL38 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPL38 Polyclonal antibody (15055-1-AP)

    IHC staining of human colon cancer using 15055-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15055-1-AP (RPL38 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RPL38 Polyclonal antibody (15055-1-AP)

    IF Staining of MCF-7 using 15055-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 15055-1-AP (RPL38 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman pancreas cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15055-1-AP targets RPL38 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL38 fusion protein Ag7038
Full Name ribosomal protein L38
Calculated Molecular Weight 8 kDa
Observed Molecular Weight 8 kDa
GenBank Accession NumberBC000603
Gene Symbol RPL38
Gene ID (NCBI) 6169
RRIDAB_10858918
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RPL38 antibody 15055-1-APDownload protocol
IHC protocol for RPL38 antibody 15055-1-APDownload protocol
IF protocol for RPL38 antibody 15055-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Oncol Rep

PP2A inhibitors suppress migration and growth of PANC-1 pancreatic cancer cells through inhibition on the Wnt/β-catenin pathway by phosphorylation and degradation of β-catenin.

Authors - Meng-Yao Wu
View Article