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RPP30 Polyclonal antibody

RPP30 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18435-1-AP

Synonyms

RNase P subunit 2, RNaseP protein p30, RNASEP2, RPP30, TSG15

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RPP30 Polyclonal antibody (18435-1-AP)

    IHC staining of human lung cancer using 18435-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18435-1-AP (RPP30 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RPP30 Polyclonal antibody (18435-1-AP)

    IHC staining of human lung cancer using 18435-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18435-1-AP (RPP30 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using RPP30 Polyclonal antibody (18435-1-AP)

    IF Staining of U-251 using 18435-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RPP30 antibody (18435-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU-251 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18435-1-AP targets RPP30 in IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPP30 fusion protein Ag8824
Full Name ribonuclease P/MRP 30kDa subunit
Calculated Molecular Weight 268 aa, 29 kDa
GenBank Accession NumberBC006991
Gene Symbol RPP30
Gene ID (NCBI) 10556
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for RPP30 antibody 18435-1-APDownload protocol
IF protocol for RPP30 antibody 18435-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols