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  • Featured Product
  • KD/KO Validated

RYK Polyclonal antibody

RYK Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22138-1-AP

Synonyms

D3S3195, JTK5, JTK5A, RYK, RYK1, Tyrosine protein kinase RYK

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using RYK Polyclonal antibody (22138-1-AP)

    WB analysis of A549 using 22138-1-AP

    WB result of RYK antibody (22138-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RYK transfected A549 cells.

  • Western Blot (WB) analysis of Jurkat cells using RYK Polyclonal antibody (22138-1-AP)

    WB analysis of Jurkat using 22138-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22138-1-AP (RYK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using RYK Polyclonal antibody (22138-1-AP)

    WB analysis of mouse brain using 22138-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22138-1-AP (RYK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using RYK Polyclonal antibody (22138-1-AP)

    WB analysis of SH-SY5Y using 22138-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22138-1-AP (RYK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using RYK Polyclonal antibody (22138-1-AP)

    IHC staining of human kidney using 22138-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 22138-1-AP (RYK antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using RYK Polyclonal antibody (22138-1-AP)

    IHC staining of human kidney using 22138-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 22138-1-AP (RYK antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using RYK Polyclonal antibody (22138-1-AP)

    IHC staining of human ovary using 22138-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 22138-1-AP (RYK antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using RYK Polyclonal antibody (22138-1-AP)

    IHC staining of human ovary using 22138-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 22138-1-AP (RYK antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using RYK Polyclonal antibody (22138-1-AP)

    IF Staining of SH-SY5Y using 22138-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 22138-1-AP (RYK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, A549 cells, mouse brain tissue, SH-SY5Y cells
Positive IHC detected inhuman kidney tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

The immunogen of 22138-1-AP is RYK Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RYK fusion protein Ag17540
Full Name RYK receptor-like tyrosine kinase
Calculated Molecular Weight 610 aa, 68 kDa
Observed Molecular Weight 67-75 kDa
GenBank Accession NumberBC153090
Gene Symbol RYK
Gene ID (NCBI) 6259
RRIDAB_11232602
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RYK antibody 22138-1-APDownload protocol
IHC protocol for RYK antibody 22138-1-APDownload protocol
IF protocol for RYK antibody 22138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Neuroinflammation

Human PMSCs-derived small extracellular vesicles alleviate neuropathic pain through miR-26a-5p/Wnt5a in SNI mice model

Authors - Yitian Lu
View Article
humanWB

Cell Mol Biol Lett

Downregulation of exosomal miR-7-5p promotes breast cancer migration and invasion by targeting RYK and participating in the atypical WNT signalling pathway

Authors - Zhaoyi Liang
View Article
humanWB

Pharmaceuticals (Basel)

RYK Gene Expression Associated with Drug Response Variation of Temozolomide and Clinical Outcomes in Glioma Patients

Authors - Ricardo D Gonzalez
View Article