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  • Featured Product
  • KD/KO Validated

RanBP2 Polyclonal antibody

RanBP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27606-1-AP

Synonyms

358 kDa nucleoporin, ANE1, E3 SUMO protein ligase RanBP2, Nucleoporin Nup358, NUP358, p270, RAN binding protein 2, RANBP2, TRP1, TRP2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RanBP2 Polyclonal antibody (27606-1-AP)

    WB analysis of HeLa using 27606-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27606-1-AP (RanBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat liver tissue using RanBP2 Polyclonal antibody (27606-1-AP)

    IHC staining of rat liver using 27606-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 27606-1-AP (RanBP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using RanBP2 Polyclonal antibody (27606-1-AP)

    IHC staining of mouse kidney using 27606-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27606-1-AP (RanBP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inrat liver tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27606-1-AP targets RanBP2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RanBP2 fusion protein Ag26370
Full Name RAN binding protein 2
Calculated Molecular Weight 358 kDa
Observed Molecular Weight 358 kDa
GenBank Accession NumberNM_006267
Gene Symbol RANBP2
Gene ID (NCBI) 5903
RRIDAB_2880923
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RanBP2 (Ran-binding protein 2) is also named as p270 and NUP358, and belongs to the RanBP2 E3 ligase family. RanBP2 is E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I (PMID:11792325, PMID:22194619). RanBP2 involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates (PMID:7775481). It is component of the nuclear export pathway (PMID:10078529). RanBP2 recruits BICD2 to the nuclear envelope and cytoplasmic stacks of nuclear pore complex known as annulate lamellae during G2 phase of cell cycle (PMID:20386726).

Protocols

Product Specific Protocols
WB protocol for RanBP2 antibody 27606-1-APDownload protocol
IHC protocol for RanBP2 antibody 27606-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Mol Biol

An Insect Viral Protein Disrupts Stress Granule Formation in Mammalian Cells

Authors - Jibin Sadasivan
View Article
humanWB,IHC

Front Oncol

YTHDF1 Aggravates the Progression of Cervical Cancer Through m6A-Mediated Up-Regulation of RANBP2.

Authors - Haocheng Wang
  • KD Validated
View Article