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SARS Polyclonal antibody

SARS Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15162-1-AP

Synonyms

SARS, Serine tRNA ligase, SERRS, SERS, seryl tRNA synthetase, Seryl tRNA(Ser/Sec) synthetase

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SARS Polyclonal antibody (15162-1-AP)

    WB analysis of HEK-293 using 15162-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15162-1-AP (SARS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SARS Polyclonal antibody (15162-1-AP)

    WB analysis of HepG2 using 15162-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15162-1-AP (SARS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SARS Polyclonal antibody (15162-1-AP)

    WB analysis of HeLa using 15162-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15162-1-AP (SARS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SARS Polyclonal antibody (15162-1-AP)

    WB analysis of HeLa using 15162-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15162-1-AP (SARS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SARS Polyclonal antibody (15162-1-AP)

    IHC staining of human lung cancer using 15162-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 15162-1-AP (SARS antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SARS Polyclonal antibody (15162-1-AP)

    IHC staining of human lung cancer using 15162-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 15162-1-AP (SARS antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SARS Polyclonal antibody (15162-1-AP)

    IF Staining of HepG2 using 15162-1-AP

    Immunofluorescent analysis of HepG2 cells using 15162-1-AP (SARS antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15162-1-AP targets SARS in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SARS fusion protein Ag7270
Full Name seryl-tRNA synthetase
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58-64 kDa
GenBank Accession NumberBC000716
Gene Symbol SARS
Gene ID (NCBI) 6301
RRIDAB_2184009
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SARS(Serine--tRNA ligase, cytoplasmic) Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).The deduced 514-amino acid protein has a calculated molecular mass of about 59 kD.And it can form a dimer of 120KDa(PMID:2180700).It is detected a band of 65KDa in MEL cell lines(PMID:1918033).

Protocols

Product Specific Protocols
WB protocol for SARS antibody 15162-1-APDownload protocol
IHC protocol for SARS antibody 15162-1-APDownload protocol
IF protocol for SARS antibody 15162-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Hematol Oncol

Integrated proteogenomic characterization of urothelial carcinoma of the bladder.

Authors - Ning Xu
View Article