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SCML1 Polyclonal antibody

SCML1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15093-1-AP

Synonyms

SCML1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCML1 Polyclonal antibody (15093-1-AP)

    WB analysis using 15093-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 15093-1-AP (SCML1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SCML1 Polyclonal antibody (15093-1-AP)

    IHC staining of mouse testis using 15093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15093-1-AP (SCML1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SCML1 Polyclonal antibody (15093-1-AP)

    IHC staining of mouse testis using 15093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15093-1-AP (SCML1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SCML1 Polyclonal antibody (15093-1-AP)

    IF Staining of HepG2 using 15093-1-AP

    Immunofluorescent analysis of HepG2 cells using 15093-1-AP (SCML1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15093-1-AP targets SCML1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCML1 fusion protein Ag5107
Full Name sex comb on midleg-like 1 (Drosophila)
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 23 kDa，34kDa
GenBank Accession NumberBC026159
Gene Symbol SCML1
Gene ID (NCBI) 6322
RRIDAB_2153243
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sex comb on midleg-like 1 (SCML1) is a meiosis-specific protein and is an essential component of the meiotic dense body. SCML1 is preferentially expressed in germ stem cells of testis, therefore likely involved in spermatogenesis.

Protocols

Product Specific Protocols
WB protocol for SCML1 antibody 15093-1-APDownload protocol
IHC protocol for SCML1 antibody 15093-1-APDownload protocol
IF protocol for SCML1 antibody 15093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols