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SCN7A-Specific Polyclonal antibody

SCN7A-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20256-1-AP

Synonyms

SCN6A, SCN7A, SCN7A-Specific

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCN7A-Specific Polyclonal antibody (20256-1-AP)

    WB analysis using 20256-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20256-1-AP (SCN7A-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SCN7A-Specific Polyclonal antibody (20256-1-AP)

    WB analysis using 20256-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 20256-1-AP (SCN7A-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using SCN7A-Specific Polyclonal antibody (20256-1-AP)

    IHC staining of mouse heart using 20256-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20256-1-AP (SCN7A-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using SCN7A-Specific Polyclonal antibody (20256-1-AP)

    IHC staining of mouse heart using 20256-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20256-1-AP (SCN7A-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, mouse heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20256-1-AP targets SCN7A-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name sodium channel, voltage-gated, type VII, alpha
Calculated Molecular Weight 193 kDa
Observed Molecular Weight 193 kDa
GenBank Accession NumberNM_002976
Gene Symbol SCN7A
Gene ID (NCBI) 6332
RRIDAB_2878660
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCN7A, also named as SCN6A, belongs to the sodium channel family. SCN7A mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, SCN7A forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. This antibody is specific to SCN7A.

Protocols

Product Specific Protocols
WB protocol for SCN7A-Specific antibody 20256-1-APDownload protocol
IHC protocol for SCN7A-Specific antibody 20256-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols