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SCRN2 Polyclonal antibody

SCRN2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11485-2-AP

Synonyms

SCRN2, secernin 2, Ses2

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using SCRN2 Polyclonal antibody (11485-2-AP)

    WB analysis of BxPC-3 using 11485-2-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11485-2-AP (SCRN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SCRN2 Polyclonal antibody (11485-2-AP)

    IHC staining of human gliomas using 11485-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11485-2-AP (SCRN2 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inBxPC-3 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11485-2-AP targets SCRN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCRN2 fusion protein Ag2001
Full Name secernin 2
Calculated Molecular Weight 46.6 kDa
Observed Molecular Weight 40-47 kDa
GenBank Accession NumberBC017317
Gene Symbol SCRN2
Gene ID (NCBI) 90507
RRIDAB_2184813
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCRN genes encode protein members of the secernin family including Secernin 1, Secernin 2 and Secernin 3. Function of Secernin 2 and Secernin 3 are so far not well understood. Secernin 1 is a novel 50-kDa cytosolic protein that appears to be involved in the regulation of exocytosis from peritoneal mast cells. Secernin-1 a novel tumor-associated antigen (TAA) and may be a universal marker of different cancer types including gastric cancer, which has been validated by several researches. Measurement of SCRN1 can be used in the early detection of cancer or in the surveillance of patients who undergo surgery.

Protocols

Product Specific Protocols
WB protocol for SCRN2 antibody 11485-2-APDownload protocol
IHC protocol for SCRN2 antibody 11485-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols