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  • Featured Product
  • KD/KO Validated

SEC11A Polyclonal antibody

SEC11A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14753-1-AP

Synonyms

1810012E07Rik, Endopeptidase SP18, SEC11 homolog A, SEC11 like protein 1, SEC11A, SEC11L1, sid2895, SPase 18 kDa subunit, SPC18, SPCS4A

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using SEC11A Polyclonal antibody (14753-1-AP)

    WB analysis of mouse testis using 14753-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14753-1-AP (SEC11A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using SEC11A Polyclonal antibody (14753-1-AP)

    WB analysis of human colon using 14753-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 14753-1-AP (SEC11A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SEC11A Polyclonal antibody (14753-1-AP)

    IHC staining of human colon cancer using 14753-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 14753-1-AP (SEC11A antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SEC11A Polyclonal antibody (14753-1-AP)

    IHC staining of human colon cancer using 14753-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 14753-1-AP (SEC11A antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inmouse testis tissue, human colon tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14753-1-AP targets SEC11A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC11A fusion protein Ag6500
Full Name SEC11 homolog A (S. cerevisiae)
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 17-21 kDa
GenBank Accession NumberBC000359
Gene Symbol SEC11A
Gene ID (NCBI) 23478
RRIDAB_2186230
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEC11A(Signal peptidase complex catalytic subunit SEC11A) is also named as SEC11L1, SPC18, SPCS4A and belongs to the peptidase S26B family. It is a component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. SEC11A has some isoforms with the molecular mass of 17-21 kDa.

Protocols

Product Specific Protocols
WB protocol for SEC11A antibody 14753-1-APDownload protocol
IHC protocol for SEC11A antibody 14753-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.

Authors - Rong Zhang
  • KO Validated
View Article
humanWB

Cell Res

Switching off IMMP2L signaling drives senescence via simultaneous metabolic alteration and blockage of cell death.

Authors - Lifeng Yuan
View Article