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SEPN1 Polyclonal antibody

SEPN1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55333-1-AP

Synonyms

MDRS1, RSMD1, RSS, SELENON, Selenoprotein N, selenoprotein N, 1, SELN, SEPN1

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using SEPN1 Polyclonal antibody (55333-1-AP)

    WB analysis of A549 using 55333-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 55333-1-AP (SEPN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using SEPN1 Polyclonal antibody (55333-1-AP)

    IHC staining of rat skeletal muscle using 55333-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse uterus tissue using SEPN1 Polyclonal antibody (55333-1-AP)

    IHC staining of mouse uterus using 55333-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse uterus tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using SEPN1 Polyclonal antibody (55333-1-AP)

    IHC staining of mouse lung using 55333-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SEPN1 Polyclonal antibody (55333-1-AP)

    IHC staining of mouse skeletal muscle using 55333-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SEPN1 Polyclonal antibody (55333-1-AP)

    IHC staining of mouse skeletal muscle using 55333-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inmouse skeletal muscle tissue, rat skeletal muscle tissue, mouse uterus tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 9 publications below

Product Information

55333-1-AP targets SEPN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name selenoprotein N, 1
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberNM_020451
Gene Symbol SEPN1
Gene ID (NCBI) 57190
RRIDAB_2881308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SEPN1 antibody 55333-1-APDownload protocol
IHC protocol for SEPN1 antibody 55333-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Front Endocrinol (Lausanne)

Changes and related factors of blood CCN1 levels in diabetic patients

Authors - Zhao-Yu Xiang
View Article
humanWB

Antioxidants (Basel)

Dietary Marginal and Excess Selenium Increased Triglycerides Deposition, Induced Endoplasmic Reticulum Stress and Differentially Influenced Selenoproteins Expression in the Anterior and Middle Intestines of Yellow Catfish Pelteobagrus fulvidraco.

Authors - Dian-Guang Zhang
View Article
mouseWB

Sci Rep

Damage to the myogenic differentiation of C2C12 cells by heat stress is associated with up-regulation of several selenoproteins.

Authors - Jiayong Tang
View Article
mouseWB

Mar Drugs

Combination of Fish Oil and Selenium Enhances Anticancer Efficacy and Targets Multiple Signaling Pathways in Anti-VEGF Agent Treated-TNBC Tumor-Bearing Mice.

Authors - Chih-Hung Guo
View Article
mouseWB

Front Nutr

Selenoprotein P Regulates Synaptic Zinc and Reduces Tau Phosphorylation.

Authors - Arlene C P Kiyohara
View Article
WB

Br J Nutr

Dietary selenium (Se) sources differentially regulate Se concentration, mRNA and protein expression of representative selenoproteins in various tissues of yellow catfish Pelteobagrus fulvidraco.

Authors - Xiao-Jian Xu
View Article