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SERPINB13 Polyclonal antibody

SERPINB13 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18045-1-AP

Synonyms

HaCaT UV repressible serpin, headpin, HSHUR7SEQ, HUR7, Hurpin, Peptidase inhibitor 13, PI 13, PI13, Proteinase inhibitor 13, Serpin B13, SERPINB13

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using SERPINB13 Polyclonal antibody (18045-1-AP)

    IHC staining of human oesophagus cancer using 18045-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18045-1-AP (SERPINB13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using SERPINB13 Polyclonal antibody (18045-1-AP)

    IHC staining of human oesophagus cancer using 18045-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18045-1-AP (SERPINB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using SERPINB13 Polyclonal antibody (18045-1-AP)

    IHC staining of mouse skin using 18045-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 18045-1-AP (SERPINB13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using SERPINB13 Polyclonal antibody (18045-1-AP)

    IHC staining of mouse skin using 18045-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 18045-1-AP (SERPINB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin tissue using SERPINB13 Polyclonal antibody (18045-1-AP)

    IHC staining of human skin using 18045-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 18045-1-AP (SERPINB13 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using SERPINB13 Polyclonal antibody (18045-1-AP)

    IHC staining of human skin using 18045-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 18045-1-AP (SERPINB13 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SERPINB13 Polyclonal antibody (18045-1-AP)

    IF Staining of HepG2 using 18045-1-AP

    Immunofluorescent analysis of HepG2 cells, using SERPINB13 antibody 18045-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SERPINB13 Polyclonal antibody (18045-1-AP)

    IF Staining of HeLa using 18045-1-AP

    Immunofluorescent analysis of Hela cells, using SERPINB13 antibody 18045-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SERPINB13 Polyclonal antibody (18045-1-AP)

    IF Staining of HeLa using 18045-1-AP

    mmunofluorescent analysis of Hela cells, using SERPINB13 antibody 18045-1-AP at 1:25 dilution and FITC-Donkey anti-Rabbit IgG (green).

Tested Applications

Positive IHC detected inhuman oesophagus cancer tissue, mouse skin tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18045-1-AP targets SERPINB13 in IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SERPINB13 fusion protein Ag12686
Full Name serpin peptidase inhibitor, clade B (ovalbumin), member 13
Calculated Molecular Weight 391 aa, 44 kDa
GenBank Accession NumberBC101821
Gene Symbol SERPINB13
Gene ID (NCBI) 5275
RRIDAB_2186160
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for SERPINB13 antibody 18045-1-APDownload protocol
IF protocol for SERPINB13 antibody 18045-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols