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  • Featured Product
  • KD/KO Validated

SERPINE2 Polyclonal antibody

SERPINE2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11303-1-AP

Synonyms

GDN, Glia derived nexin, Peptidase inhibitor 7, PI 7, PI7, PN 1, PN1, PNI, Protease nexin 1, Protease nexin I, Serpin E2, SERPINE2

Validation Data Gallery

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  • Western Blot (WB) analysis of C6 cells using SERPINE2 Polyclonal antibody (11303-1-AP)

    WB analysis of C6 using 11303-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using SERPINE2 Polyclonal antibody (11303-1-AP)

    WB analysis of rat heart using 11303-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SERPINE2 Polyclonal antibody (11303-1-AP)

    WB analysis of MCF-7 using 11303-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using SERPINE2 Polyclonal antibody (11303-1-AP)

    WB analysis of A549 using 11303-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using SERPINE2 Polyclonal antibody (11303-1-AP)

    WB analysis of rat brain using 11303-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using SERPINE2 Polyclonal antibody (11303-1-AP)

    IP experiment of A549 using 11303-1-AP

    IP result of anti-SERPINE2 (IP:11303-1-AP, 3ug; Detection:11303-1-AP 1:300) with A549 cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using SERPINE2 Polyclonal antibody (11303-1-AP)

    IHC staining of human testis using 11303-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 11303-1-AP (SERPINE2 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human testis tissue using SERPINE2 Polyclonal antibody (11303-1-AP)

    IHC staining of human testis using 11303-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 11303-1-AP (SERPINE2 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SERPINE2 Polyclonal antibody (11303-1-AP)

    IHC staining of human pancreas cancer using 11303-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11303-1-AP (SERPINE2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of human testis tissue using SERPINE2 Polyclonal antibody (11303-1-AP)

    IF Staining of human testis using 11303-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human testis tissue using SERPINE2 antibody (11303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human testis tissue using SERPINE2 Polyclonal antibody (11303-1-AP)

    IF Staining of human testis using 11303-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human testis tissue using SERPINE2 antibody (11303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using SERPINE2 Polyclonal antibody (11303-1-AP)

    IF Staining of A549 using 11303-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SERPINE2 antibody (11303-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using SERPINE2 Polyclonal antibody (11303-1-AP)

    IF Staining of mouse testis using 11303-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SERPINE2 antibody (11303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inC6 cells, A549 cells, MCF-7 cells, rat brain tissue, rat heart tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman testis tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman testis tissue, A549 cells, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11303-1-AP targets SERPINE2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SERPINE2 fusion protein Ag1830
Full Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44-50 kDa
GenBank Accession NumberBC015663
Gene Symbol SERPINE2
Gene ID (NCBI) 5270
RRIDAB_10732939
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.

Protocols

Product Specific Protocols
WB protocol for SERPINE2 antibody 11303-1-APDownload protocol
IHC protocol for SERPINE2 antibody 11303-1-APDownload protocol
IF protocol for SERPINE2 antibody 11303-1-APDownload protocol
IP protocol for SERPINE2 antibody 11303-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IP

Cancer Lett

SERPINE2/PN-1 regulates the DNA damage response and radioresistance by activating ATM in lung cancer.

Authors - Jingjing Zhang
  • KD Validated
View Article
humanWB,IHC

Respir Res

High expression level of serpin peptidase inhibitor clade E member 2 is associated with poor prognosis in lung adenocarcinoma.

Authors - Ryota Dokuni
  • KD Validated
View Article
humanWB

J Immunol

Deep Coverage Tissue and Cellular Proteomics Revealed IL-1β Can Independently Induce the Secretion of TNF-Associated Proteins from Human Synoviocytes.

Authors - Shengquan Tang
View Article
humanIP

Front Cell Dev Biol

BAP31 Promotes Tumor Cell Proliferation by Stabilizing SERPINE2 in Hepatocellular Carcinoma.

Authors - Xiyang Zhang
View Article
mouseIF

Sci Rep

PMID: 30446696

Authors - Mary Ann Stepp
View Article
humanIHC

J Cell Physiol

Transcriptional profiling of endometriosis tissues identifies genes related to organogenesis defects.

Authors - Crispi Stefania S
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Ahmad (Verified Customer) (06-18-2021)

Fix in 1x PBS, 1% formaldehyde, 2 mM MgCl2, 5 mM EGTA, 0.02% NP-40 for 1 hr works good for staining

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: rabbit cornea
SERPINE2 Antibody Immunofluorescence validation (1:300 dilution) in rabbit cornea (Cat no:11303-1-AP)