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SERPING1/C1 Inactivator Polyclonal antibody

SERPING1/C1 Inactivator Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12259-1-AP

Synonyms

C1 esterase inhibitor, C1 Inh, C1 inhibiting factor, C1IN, C1INH, C1NH, HAE1, HAE2, Plasma protease C1 inhibitor, Serpin G1, SERPING1, SERPING1/C1 Inactivator

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SERPING1/C1 Inactivator Polyclonal antibody (12259-1-AP)

    WB analysis using 12259-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SERPING1/C1 Inactivator Polyclonal antibody (12259-1-AP)

    WB analysis of HeLa using 12259-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using SERPING1/C1 Inactivator Polyclonal antibody (12259-1-AP)

    WB analysis of human liver using 12259-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using SERPING1/C1 Inactivator Polyclonal antibody (12259-1-AP)

    WB analysis of L02 using 12259-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SERPING1/C1 Inactivator Polyclonal antibody (12259-1-AP)

    WB analysis of mouse liver using 12259-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SERPING1/C1 Inactivator Polyclonal antibody (12259-1-AP)

    WB analysis of HepG2 using 12259-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SERPING1/C1 Inactivator Polyclonal antibody (12259-1-AP)

    WB analysis of mouse liver using 12259-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SERPING1/C1 Inactivator Polyclonal antibody (12259-1-AP)

    IHC staining of human tonsillitis using 12259-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SERPING1/C1 Inactivator Polyclonal antibody (12259-1-AP)

    IHC staining of human tonsillitis using 12259-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SERPING1/C1 Inactivator Polyclonal antibody (12259-1-AP)

    IF Staining of HepG2 using 12259-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SERPING1/C1 Inactivator antibody (12259-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SERPING1/C1 Inactivator Polyclonal antibody (12259-1-AP)

    IF Staining of HepG2 using 12259-1-AP

    Immunofluorescent analysis of HepG2 cells using 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman plasma, HeLa cells, human liver tissue, L02 cells, mouse liver tissue, HepG2 cells, human urine tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12259-1-AP targets SERPING1/C1 Inactivator in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SERPING1/C1 Inactivator fusion protein Ag2819
Full Name serpin peptidase inhibitor, clade G (C1 inhibitor), member 1
Calculated Molecular Weight 500 aa, 55 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC011171
Gene Symbol SERPING1
Gene ID (NCBI) 710
RRIDAB_2285663
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPING1, also known as C1 Inhibitor (C1INH), is a member of the Serine proteinase inhibitor family. Its main function is the inhibition of the complement system to prevent spontaneous activation. It inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE). C1INH is the most heavily glycosylated plasma protein. Of its 95-105 kDa apparent molecular mass, the protein moiety of 500 aa accounts for only 55 kDa. The native 105 kDa form of C1INH could be cleaved into 60-65 kDa fragments.

Protocols

Product Specific Protocols
WB protocol for SERPING1/C1 Inactivator antibody 12259-1-APDownload protocol
IHC protocol for SERPING1/C1 Inactivator antibody 12259-1-APDownload protocol
IF protocol for SERPING1/C1 Inactivator antibody 12259-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Glia

Knockdown of circulating C1 inhibitor induces neurovascular impairment, glial cell activation, neuroinflammation, and behavioral deficits.

Authors - Dorit Farfara
View Article
mouseIF

J Neuroinflammation

Fluoxetine inhibited the activation of A1 reactive astrocyte in a mouse model of major depressive disorder through astrocytic 5-HT2BR/β-arrestin2 pathway.

Authors - Yinquan Fang
View Article
humanWB

J Thromb Haemost

The Alzheimer's disease peptide Aβ promotes thrombin generation through activation of coagulation factor XII.

Authors - Daria Zamolodchikov
View Article
mouseWB

J Thromb Haemost

The Alzheimer's disease peptide β-amyloid promotes thrombin generation through activation of coagulation factor XII: reply.

Authors - D Zamolodchikov
View Article
humanIHC

J Alzheimers Dis

A New Serum Biomarker Set to Detect Mild Cognitive Impairment and Alzheimer's Disease by Peptidome Technology.

Authors - Koji Abe
View Article
humanIHC

Urol Oncol

Decreased expression of serine protease inhibitor family G1 (SERPING1) in prostate cancer can help distinguish high-risk prostate cancer and predicts malignant progression.

Authors - Shengmeng Peng
View Article