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SETMAR Polyclonal antibody

SETMAR Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25814-1-AP

Synonyms

HsMar1, METNASE, SETMAR

Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using SETMAR Polyclonal antibody (25814-1-AP)

    WB analysis of HL-60 using 25814-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 25814-1-AP (SETMAR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SETMAR Polyclonal antibody (25814-1-AP)

    WB analysis using 25814-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25814-1-AP (SETMAR antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using SETMAR Polyclonal antibody (25814-1-AP)

    WB analysis of L02 using 25814-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 25814-1-AP (SETMAR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HL-60 cells using SETMAR Polyclonal antibody (25814-1-AP)

    IP experiment of HL-60 using 25814-1-AP

    IP result of anti-SETMAR (IP:25814-1-AP, 4ug; Detection:25814-1-AP 1:500) with HL-60 cells lysate 1600ug.

Tested Applications

Positive WB detected inHL-60 cells, L02 cells, HEK-293T cells, Jurkat cells
Positive IP detected inHL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25814-1-AP is SETMAR Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SETMAR fusion protein Ag22938
Full Name SET domain and mariner transposase fusion gene
Calculated Molecular Weight 671 aa, 77 kDa
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC008931
Gene Symbol SETMAR
Gene ID (NCBI) 6419
RRIDAB_2880248
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SETMAR antibody 25814-1-APDownload protocol
IP protocol for SETMAR antibody 25814-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Structural and genome-wide analyses suggest that transposon-derived protein SETMAR alters transcription and splicing.

Authors - Qiujia Chen
View Article