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SH2D5 Polyclonal antibody

SH2D5 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55320-1-AP

Synonyms

SH2 domain containing 5, SH2D5

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SH2D5 Polyclonal antibody (55320-1-AP)

    WB analysis of HEK-293 using 55320-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55320-1-AP (SH2D5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using SH2D5 Polyclonal antibody (55320-1-AP)

    WB analysis of mouse kidney using 55320-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55320-1-AP (SH2D5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using SH2D5 Polyclonal antibody (55320-1-AP)

    IHC staining of human cerebellum using 55320-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using SH2D5 Polyclonal antibody (55320-1-AP)

    IHC staining of human cerebellum using 55320-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using SH2D5 Polyclonal antibody (55320-1-AP)

    IHC staining of human brain using 55320-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using SH2D5 Polyclonal antibody (55320-1-AP)

    IHC staining of human brain using 55320-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SH2D5 Polyclonal antibody (55320-1-AP)

    IF Staining of HEK-293 using 55320-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SH2D5 antibody (55320-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IHC detected inhuman cerebellum tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55320-1-AP targets SH2D5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name SH2 domain containing 5
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 43-47 kDa
GenBank Accession NumberNM_001103160
Gene Symbol SH2D5
Gene ID (NCBI) 400745
RRIDAB_2881307
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SH2D5 antibody 55320-1-APDownload protocol
IHC protocol for SH2D5 antibody 55320-1-APDownload protocol
IF protocol for SH2D5 antibody 55320-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols