Siglec-7 Polyclonal antibody
Siglec-7 Polyclonal Antibody for WB, FC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF, FC, IHC, ELISA
Conjugate
Unconjugated
Cat no : 13939-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | U-937 cells, A549 cells, human liver tissue, human placenta tissue, K-562 cells, L02 cells |
| Positive IHC detected in | human tonsillitis tissue, human liver tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | U-937 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
13939-1-AP targets Siglec-7 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Siglec-7 fusion protein Ag4220 |
| Full Name | sialic acid binding Ig-like lectin 7 |
| Calculated Molecular Weight | 467 aa, 51 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC028150 |
| Gene Symbol | SIGLEC7 |
| Gene ID (NCBI) | 27036 |
| RRID | AB_10919158 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Sialic acid binding Ig-like lectin 7 (Siglec-7), also known as CD328 or p75/AIRM-1, is a member of the Siglec family of glycan-recognition proteins. Siglec-7 is a 75-kDa type-I transmembrane protein consisting of three extracellular immunoglobulin-like domains that comprise an N-terminal V-set domain and two C2-set domains, a transmembrane region and a cytoplasmic tail containing two tyrosine residues embodied in immunoreceptor tyrosine-based inhibition motif-like motifs (PMID: 32322597; 10567377). It is mainly expressed on immune cells, with low levels on granulocytes, intermediate levels on monocytes, and relatively high levels on a major subset of natural killer cells and a minor subset of CD8+ T cells (PMID: 10567377). Siglec-7 is an inhibitory receptor that negatively regulates the function of NK cells and modulates the immune response through the interaction of sialic acid-containing ligands (PMID: 27312286).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Siglec-7 antibody 13939-1-AP | Download protocol |
| IHC protocol for Siglec-7 antibody 13939-1-AP | Download protocol |
| FC protocol for Siglec-7 antibody 13939-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncol Lett Siglec-7 is a predictive biomarker for the efficacy of cancer vaccination against metastatic colorectal cancer. | ||
Commun Biol ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3Gal1) synthesis of Siglec ligands mediates anti-tumour immunity in prostate cancer |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Emma (Verified Customer) (11-29-2021) | Works well by IF on FFPE tissue. Antigen retrieval Tris-EDTA pH 9.
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