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SLAM/CD150 Polyclonal antibody

SLAM/CD150 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 10949-2-AP

Synonyms

CD150, CDw150, IPO 3, SLAM, SLAMF1

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using SLAM/CD150 Polyclonal antibody (10949-2-AP)

    WB analysis of Jurkat using 10949-2-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10949-2-AP (SLAM/CD150 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10949-2-AP targets SLAM/CD150 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLAM/CD150 fusion protein Ag1386
Full Name signaling lymphocytic activation molecule family member 1
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC012602
Gene Symbol SLAMF1
Gene ID (NCBI) 6504
RRIDAB_2187822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signaling lymphocytic activation molecule (SLAM, also known as SLAMF1 or CD150) is a 70-95 kDa transmembrane glycoprotein that belongs to the immunoglobulin gene superfamily. SLAM is constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. SLAM is involved in T cell stimulation and cytokine production, and may play an important role in the regulation of immune responses to pathogens.

Protocols

Product Specific Protocols
WB protocol for SLAM/CD150 antibody 10949-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols