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  • Featured Product
  • KD/KO Validated

SLMAP Polyclonal antibody

SLMAP Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25220-1-AP

Synonyms

KIAA1601, sarcolemma associated protein, Sarcolemmal associated protein, SLAP, SLMAP

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using SLMAP Polyclonal antibody (25220-1-AP)

    WB analysis of mouse heart using 25220-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25220-1-AP (SLMAP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using SLMAP Polyclonal antibody (25220-1-AP)

    WB analysis of mouse skeletal muscle using 25220-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25220-1-AP (SLMAP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using SLMAP Polyclonal antibody (25220-1-AP)

    WB analysis of A431 using 25220-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 25220-1-AP (SLMAP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse heart tissue, A431 cells, mouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25220-1-AP is SLMAP Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLMAP fusion protein Ag18162
Full Name sarcolemma associated protein
Calculated Molecular Weight 828 aa, 95 kDa
Observed Molecular Weight 35 kDa, 45 kDa, 63-66 kDa, 83-91 kDa
GenBank Accession NumberBC115701
Gene Symbol SLMAP
Gene ID (NCBI) 7871
RRIDAB_2879968
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sarcolemmal membrane associated protein (SLMAP) is a tail-anchored membrane protein involved in the docking and membrane fusion process via protein-protein interactions. Deregulation of SLMAP expression plays an important role in type 2 diabetes, and changes in SLMAP expression are linked to hyperglycemia and diabetes. SLMAPs are components of cardiac membranes and have been identified as a novel regulator of cardiac function. There are several isoforms of SLMAPs with varying molecular size (35, 45, 63, and 83-91 kDa) as a result of alternative splicing. The short 35-kDa isoform is predominantly expressed in cardiac and slow-twitch skeletal muscle, while the 83- to 91-kDa isoform is ubiquitous. This antibody recognizes all of these isoforms in mouse heart lysate. (21785580, 22180652)

Protocols

Product Specific Protocols
WB protocol for SLMAP antibody 25220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Thorac Cancer

Tumor suppressor effect of an antibody on xenotransplanted sarcomatoid mesothelioma cells

Authors - Masayoshi Hasegawa
  • KD Validated
View Article